12/31/09
- ✓ Minipreps: KAH117(2), 118(2), 119(4); KAH94, 95/JDS43(6 each)
- ✓ Cultures: KAH117 (4)
Minipreps
> Check KAH117-119 w/ E/P
> Check KAH94, 95/JDS43 w/ PstI
Reagent |
1-8 |
9-20
|
Expected: 1,2. KAH117 = 7758 3,4. KAH118 = 7930 5-8. KAH119 = 8002 9-14. KAH94 fwd = ~8000, 2000, 1800, 500 KAH94 rev = ~5500, 3000, 2000, 1800 15-20. KAH95 fwd = ~8000, 2000, 1800, 500 KAH95 rev = ~5500, 3000, 2000, 1800
|
15 μL/lane; 1% agarose
|
DNA(plasmid) |
4.0 |
4.0
|
10X buffer |
1.5 |
1.5
|
EcoRI |
1.0 |
---
|
PstI |
1.0 |
2.0
|
dH2O |
7.5 |
7.5
|
|
15 μL |
15 μL
|
--> 37°C/ ~30 min.
--> Pick 4 more colonies for KAH117 (keep 2 for re-digestion; correct size but low yield?)
--> Try re-cloning KAH94, 95/ JDS43(and /JDS42) later
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