User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/31

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12/31/09

  • ✓ Minipreps: KAH117(2), 118(2), 119(4); KAH94, 95/JDS43(6 each)
  • ✓ Cultures: KAH117 (4)

Minipreps
> Check KAH117-119 w/ E/P
> Check KAH94, 95/JDS43 w/ PstI

Reagent 1-8 9-20 Expected:
1,2. KAH117 = 7758
3,4. KAH118 = 7930
5-8. KAH119 = 8002
9-14. KAH94 fwd = ~8000, 2000, 1800, 500
KAH94 rev = ~5500, 3000, 2000, 1800
15-20. KAH95 fwd = ~8000, 2000, 1800, 500
KAH95 rev = ~5500, 3000, 2000, 1800
File:KAH 123109 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 4.0 4.0
10X buffer 1.5 1.5
EcoRI 1.0 ---
PstI 1.0 2.0
dH2O 7.5 7.5
  15 μL 15 μL

--> 37°C/ ~30 min.
--> Pick 4 more colonies for KAH117 (keep 2 for re-digestion; correct size but low yield?) --> Try re-cloning KAH94, 95/ JDS43(and /JDS42) later