User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/11

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12/11/09 ✓ Minipreps: KAH120-125 (3 each, 18 total) ✓ Cultures: KAH126-134 (2 each, 18 total) Minipreps > Check with E/P digests Reagent Volume Expected: 1-3. KAH120 = 7930 4-6. KAH121 = 8102 7-9. KAH122 = 8174 10-12. KAH123 = 8002 13-15. KAH124 = 8174 16-18. KAH125 = 8246 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min. --> Pick more KAH123-125 colonies