User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/11

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

12/11/09

  • ✓ Minipreps: KAH120-125 (3 each, 18 total)
  • ✓ Cultures: KAH126-134 (2 each, 18 total)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
1-3. KAH120 = 7930
4-6. KAH121 = 8102
7-9. KAH122 = 8174
10-12. KAH123 = 8002
13-15. KAH124 = 8174
16-18. KAH125 = 8246
File:KAH 121109 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL

--> 37°C/ ~15 min.

--> Pick more KAH123-125 colonies