User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/04

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12/04/09 ✓ Minipreps: KAH93(2), 94(2), 95/MV2(2) ✓ Cultures: KAH99(2), 100(2), 101(2), 105(2), 106(2), 107(2), 108/MV2(2) ✓ Check sequencing results for KAH011 (NLS-myc-stop) Minipreps > Check with E/P/ScaI digests Reagent Volume Expected: 1,2. KAH93/MV2 = 4839 3,4. KAH94/MV2 = 5011 5,6. KAH95/MV2 = 5083 MV2 fragments = 3811, 709 15 μL/lane; 1% agarose Confirm by sequencing. Pick 2 more clones for KAH95/MV2. DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 ScaI 1.0 dH2O 8.5   15 μL --> 37°C/ ~15 min. Sequencing results KAH011-1: bad clone KAH011-2: good ✓ (Keep) KAH011-3: good ✓ KAH011-4: good ✓ KAH011-5: good ✓ KAH011-6: good ✓ KAH011-7: good ✓ KAH011-8: good ✓