User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/03

iGEM 2011

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Objectives Transformation of Ligation BBa_P1003 + BBa_B0015(double ter) in pSB1AK3. Plasmid extraction of pSB1C3(A3 plate 1 dist 2011) and pSB1AK3/BBa_B0015 (backbone and double ter). Transformation Was performed with yesterday´s ligation in DH5α E.Coli. I hope kanamycin resistance, by backbone and I need a intesive search for false positives and I have control for it. Method:Miguel´s Lab method. Plasmid extraction Miguel´s Lab method (miniprep) Lane 1-3 pSB1C3 Lane 4-6 pSB1AK3/BBa_B0015. I will cut with XhoI pSB1C3, for pSB1AK3/BBa_B0015 I have previously cut