User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/02

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Objectives

  • Ligation of Plasmid pSB1AK3/BBa_B0015 and Prom+Kanamycin.

Ligation

5:2 4:3 -Vector -Insert
DNA insert 5 4 5 0
DNA vector 2 3 0 3
Buffer T4 DNA Ligase NEB 1 1 1 1
T4 DNA Ligase NEB 1 1 0 0
H2O 1 1 4 6

I left reaction overnight. -Vector and -Insert are my controls to see right results.