User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/09

iGEM 2011

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Objective Clean and know purify of Daniel´s PCR WetLab Purification of PCR product with Roche´s kit of High Pure PCR product. I joined tube A and B and were filled to 100μL See purity of PCR cleaning with a gel. Lane 1.5μL Ladder Lane 2.3μL dye & 5μL DNA of cleaned PCR. Lane 3.3μL dye & 5μL Control (Tube C from Daniel´s extraction). Ligation of PCR purification: I made two ligations by distinct ways. DNA insert 5μL DNA vector 1μL Buffer10X 1μL H2O 1μL T4LigaseNEB 1μL Second one DNA insert 2μL DNA vector 3μL Buffer10X 1μL H2O 3μL T4LigaseNEB 1μL