Illuminating evolution one compartment at a time. Pioneering spatial proteomics approaches in photosynthetic microeukaryotes at Harvard University, the Scripps Institution of Oceanography, and the J. Craig Venter Institute (JCVI).
Be part of my efforts to save coral reefs at Let’s all tri our best, TriForOceans!
On Sep 1, 2016, I became a Visiting Graduate Student in Prof. Andrew E. Allen Lab at the Scripps Institution of Oceanography (SIO) and the J. Craig Venter Institute (JCVI) where I am continuing my spatial proteomics work in diatoms. My new mailing address is as follows:
J. Craig Venter Institute
4120 Capricorn Lane
La Jolla, CA 92037
Email: email@example.com & firstname.lastname@example.org
Molecular and cell biologist with broad international academic and industrial research experience. PhD Candidate in Biological and Biomedical Sciences at Harvard University (expected to graduate in Spring 2019), Visiting graduate student at University of California, San Diego, and Research Fellow at J. Craig Venter Institute. iGEM 2010 world champion and protocols.io ambassador. Open science advocate.
In my PhD thesis I am using a genetically encodable tag for subcellular proteomics to study endocytosis, carbon fixation and biosilicification in diatoms, highly abundant and evolutionarily successful photosynthetic microeukaryotes.
My future interests with tangents to my PhD work are as follows:
1. Continuing my work on systems understanding of the molecular mechanisms guiding the morphogenesis of diatom cell walls. I am particularly interested in polyamines and their role in connecting cell cycle control and call wall formation.
2. Extending my studies on biomineral morphogenesis to non-diatom model systems such as radiolarian protozoa Acantharea which are using strontium sulfate to build their skeletons.
3. Studying evolutionary origins and basic physicochemical principles of protein-induced phase transitions in eukaryotes. I am especially interested in how protein disorder, low amino acid complexity and posttranslational modifications tie to these processes.
4. Investigating antizyme-mediated regulation of polyamine homeostasis and the mechanisms of polyamine-induced ribosomal frameshifting.
I have always been very motivated to balance science with my passion for sports which will extend into all of my future endeavors. I have developed as a triathlete throughout graduate school and am anticipating to participate in my first full Ironman race in 2019.
- 1 Contact Info
- 2 Interests
- 3 Projects
- 4 Funding
- 5 Peer reviewed publications
- 6 Open source publications
- 7 Other publications
- 8 Creative writing
- 9 Conferences
- 9.1 A New Age of Discovery for Aquatic Microeukrayotes, 26-29 Januar 2016, EMBL Heidelberg, Germany
- 9.2 The Eleventh Annual Broad Institute Retreat, 14-15 December 2015, Hynes Convention Center, Boston, MA
- 9.3 Wyss Institute 7th Annual Retreat, 19 November 2015, Seaport World Trade Center, Boston, MA
- 9.4 Molecular Life of Diatoms 2015, 7-10 July 2015, University of Washington, Seattle, WA
- 10 Talks
- 11 Teaching
- 12 Outreach
- 13 Past Affiliations
- 14 Selected Awards & Accomplishments
- 15 Education
- 16 Sports
- 17 Podcasts
- 18 Web
- Jernej Turnšek | Ph.D. Candidate
- Harvard Medical School | Department of Systems Biology | 200 Longwood Avenue, Boston, MA 02115
- Wyss Institute for Biologically Inspired Engineering at Harvard University | Center for Life Science Boston Bldg., 5th Floor | 3 Blackfan Circle, Boston, MA 02115
- Phone: (617) 797-5386 | Fax: (617) 432-5012 | Email: email@example.com | CV as of July 16'
- Email me through OpenWetWare
- Biomineralization | Biomaterials | Morphogenesis | Self-Assembly | Biomolecular condensates and biological phase transitions
- Marine Microbiome | Marine Natural Products
- Astrobiology | Space Exploration | Origins of Life
- Endurance Sports | Exercise Physiology | Sports Technology
- Spatial biosilicification-specific proteomics in the model diatom Thalassiosira pseudonana.
Figure 1 | Overview of the biosilicification process in diatoms. (Left) Diatom cell wall is synthesized inside an acidic compartment called the silica deposition vesicle (SDV). (Right) It is hypothesized that the interaction of silaffins with long-chain polyamines (LCPA) inside the SDV leads to the formation of an organic matrix, the structure of which is controlled by the silaffins (adapted from Kröger, 2007, and Kröger & Poulsen, 2008).
- N. Kröger, Prescribing diatom morphology: toward genetic engineering of biological nanomaterials. Curr. Opin. Chem. Biol. 11, 662–669 (2007).
- N. Kröger, N. Poulsen, Diatoms—From Cell Wall Biogenesis to Nanotechnology. Annu. Rev. Genet. 42, 83–107 (2008).
- M. Hildebrand, S. J. L. Lerch, Diatom silica biomineralization: Parallel development of approaches and understanding. Semin. Cell Dev. Biol. 46, 27–35 (2015).
Figure 2 [A] Morphological diversity of diatom cell walls. The image represents cleaned diatom cell walls arranged in the star-like pattern by the UK-based microscopist and diatom artist Klaus Kemp. [B] SEM image of Thalassiosira pseudonana, the model diatom for biomineralization studies (credit: Nils Kröger). [C] Thalassiosira pseudonana cells. Red and green correspond to plastid autofluorescence and silica, respectively.
See poster links under the "Conferences" section for more details.
My work is supported by the Gordon and Betty Moore Foundation: "The Gordon and Betty Moore Foundation believes in bold ideas that create enduring impact in the areas of science, environmental conservation and patient care. Intel co-founder Gordon and his wife Betty established the foundation to create positive change around the world and at home in the San Francisco Bay Area. Science looks for opportunities to transform–or even create–entire fields by investing in early-stage research, emerging fields and top research scientists. Environmental conservation efforts promote sustainability, protect critical ecological systems and align conservation needs with human development. Patient care focuses on eliminating preventable harms and unnecessary healthcare costs through meaningful engagement of patients and their families in a supportive, redesigned healthcare system." Visit Moore.org or follow @MooreFound and read more about the broader research initiative my projects fall under on the following link.
Peer reviewed publications
- R. J. Conrado et al., DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency. Nucleic Acids Res. 40, 1879–1889 (2012).
Open source publications
- Revisited Thalassiosira pseudonana (Tp) conjugation protocol enables fusion protein delivery to Tp frustule (protocols.io, Feb 18, 2018)
- Vacuole staining in Phaeodactylum tricornutum using RatioWorks™ PDMPO (protocols.io, Feb 18, 2018)
- HA tag enables highly efficient detection of heterologous proteins in Phaeodactylum tricornutum (Pt) exconjugants (protocols.io, Oct 5, 2017)
- Guidelines for highly efficient construction of diatom episomes using Gibson Assembly (protocols.io, Sep 21, 2017)
- Pour plating of Thalassiosira pseudonana (Tp) (protocols.io, Aug 16, 2017)
- Simple & rapid genotyping of marine microeukaryotes (protocols.io, Aug 11, 2017)
- Conjugation of Thalassiosira pseudonana (protocols.io, Oct 19, 2016)
A New Age of Discovery for Aquatic Microeukrayotes, 26-29 Januar 2016, EMBL Heidelberg, Germany
This conference celebrated efforts of three major contributions to microeukaryote biology, ecology, and evolution that are catapulting the field forward: the Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP), the Tara Oceans Expeditions, and the Malaspina Expedition. You can find the poster I presented at the conference on this link.
The Eleventh Annual Broad Institute Retreat, 14-15 December 2015, Hynes Convention Center, Boston, MA
This event brings together researchers and affiliates of the Broad Institute to discuss institute's most recent advances at the interface of science and technology. You can find the poster I presented at the retreat here
Wyss Institute 7th Annual Retreat, 19 November 2015, Seaport World Trade Center, Boston, MA
This event brings together researchers and affiliates of the Wyss Institute for Biologically Inspired Engineering to discuss institute's most recent advances at the interface of science and technology. You can find the poster I presented at the retreat on this link.
Molecular Life of Diatoms 2015, 7-10 July 2015, University of Washington, Seattle, WA
Molecular Life of Diatoms 2015 brought together an interdisciplinary mix of scientists to discuss recent advances in our understanding of diatoms and the way they shape our planet. You can find the poster I presented at the conference here.
- SIO262 - Seminars in Marine Chemical Biology and Natural Products, Scripps Insitution of Oceanography, 11/01/2017
- Synthetic Biology Platform Meeting, Wyss Institute for Biologically Inspired Engineering, 03/01/2016 | slides
- Mentoring a B.S. level scientist, JCVI, CA, April 17'-now
Molecular cloning & microalgae genetic engineering.
- Biochemistry Bootcamp Mentor, Wellesley College, Wellesley, MA, January 2015
I served as a mentor for the inaugural biochemistry bootcamp aimed at promising undergraduates at Wellesley College. Students expressed a protein in bacteria, purified it, assayed its activity, and presented their findings to their classmates. I directly supervised a pair of undergraduate participants. The administrators at Wellesley agreed to offer this bootcamp opportunity to students again in 2016.
My PhD project in plain English
- 3 slides describing my PhD project in simple terms can be found here.
SITN Waves Correspondent
SITN Waves Expert
- Friend or Foe? Getting the body to make peace with bioengineered drug delivery systems
- Algae: Applications in Removing Arsenic and Beyond
- Can we send a message to the future with DNA?
- Electric Bacteria – Groundbreaking Science or Old News?
- Scientists Learn to 3D Print Cells One Drop at A Time
In the news
- "Successful first seven decades of Ljubljana's Biotechnical Faculty", February 2017 (in Slovene)
- MetaPHoDcast, January 2016 (in Slovene)
- Wyss Institute, December 2015
- Finance, February 2015 (in Slovene)
- Ginkgo BioWorks, Inc., Boston, MA (10/2012 - 06/2013) | Ginkgo BioWorks
- Jerala Lab at the National Institute of Chemistry, Ljubljana, Slovenia (05/2010 - 07/2012) | Jerala Lab
Selected Awards & Accomplishments
- Kavčič Prize for young writers in natural sciences, 2013
- University of Ljubljana Prešeren Award for Diploma Thesis, 2012 (in Slovene)
- 42nd Krka Prizes - Krka Prize for Diploma Thesis, 2012 (in Slovene)
- Gold medal, BIOMOD 2011 | Learn more about BIOMOD Slovenia 2011
- Grand Prize, World Champion, iGEM 2010 | Learn more about iGEM Slovenia 2010
- University of Ljubljana, Biotechnical Faculty, Diploma in Biotechnology, 06/2012
- Diploma thesis entitled: "Synthetic biology approach towards improvement of carotenoid biosynthetic pathway using zinc fingers"
- Swami's Cycling Club
- Triathlon Club of San Diego
- UC San Diego Cycling Team
- La Jolla Cove Swim Club
- MIT Triathlon Club
- Harvard University Cycling Association
- Harvard Triathlon Team
- November Project
- 70.3 Ironman triathlon PR | Timberman 2016 08/2016: 5h 24' 02"
You can find reflections on my first Ironman 70.3 journey on this link.
- Olympic distance triathlon PR | Mass State Triathlon 07/2016: 2h 17' 24"
- Sprint distance triathlon PR | Mission Bay Triathlon 10/2017: 58' 33"
- 26.2 PR | Baystate Marathon 10/2013: 3h 30' 55"
- 13.1 PR | Boston's Run to Remember 05/2013: 1h 26' 30"
- 10K PR | Mardi Gras 10K 02/2017: 38' 26"
- 5K PR | Triton 5K 04/2017: 18' 32"
- University of Ljubljana basketball champion in 2008 and 2009.
Science, Technology, Entertainment, Design
- Chemistry World | Disruptive | Discovery | Nature | Nature Biotechnology | NPR: Invisibilia | NPR: Ted Radio Hour | Science Magazine | Science Talk | Science Weekly | SIT'N Listen! | StarTalk Radio | Stuff You Should Now | The Guardian's Science Weekly | WNYC's Radiolab
- Ask a Cycling Coach | Endurance Nation | Over The Top Cycling | Running Rogue | The Kona Edge | The Rich Roll Podcast | TOWER26 | Trail Runner Nation | Zen and The Art of Triathlon | 220 Triathlon Magazine
- Apparatus | Frekvenca X | Intelekta | Meta PHoDcast | Podrobnosti | Studio ob 17h | Stevilke | Vroci Mikrofon
- Documentaries | Fresh Air | Hello PhD | The Coffee Podcast | The 5AM Miracle Podcast | Vinyl Schminyl Radio
- Tara Oceans | The Secret Life of Plankton | Five Reasons to Thank Plankton | Ocean Drifters | The Diatomist
Science, Technology, Entertainment, Design
- Compound Interest | Khan Academy | ScienceDaily | Selected Lectures on Science and Engineering in the Boston Area | TED