User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/09/14

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Today's objective is to cross link the film created on Friday and to recreate clay beads using 10% clay composite.


Bead Synthesis
The full procedure of the large scale synthesis can be found on Mary's notebook entry.

  • A solution of 8.33mL 3% wt bentonite solution, 13.64mL of DI water, and 8.03mL of concentrated HCl (to create 30 mL of a 10% HCl in clay solution) was heated and stirred until all PVA beads were dissolved.
  • The solution was then transferred to a burette.
  • 10mL of the PVA-clay solution was added, dropwise, to 100mL of ethyl acetate, while stirring vigorously.
  • 2mL of glutaraldehyde was added and the solution was stirred for 7 minutes.
  • After 7 minutes, 7mL of sodium bicarbonate solution was added and stirred for 30 seconds.
  • The solution was removed and put into 50mL Falcon tubes.
  • The solution was centrifuged for 1 minutes at 3000 rpm.
  • The liquid was pipetted out of the tubes and replaced with DI water to store.

Cross-linking films
Film cross-linking was preformed using the procedure found here.


In the past, there was not enough ethyl acetate to fully perform the bead synthesis, likely due to the surface area being large and the spinning helping to evaporate the ethyl acetate faster than the bead synthesis takes place. Instead, we doubled the volumes in this procedure (compared to the last) from 50mL of ethyl acetate and 1mL of glutaraldehyde to 100mL ethyl acetate and 2mL glutaraldehyde.
Good use of links.