Determine the molar absorptivity of adenosine and inosine.
Creating Stock Solutions
- Massed a .0817 gram sample of adenosine and a .1261 g sample of inosine
- Placed both samples into separate 100 mL volumetric flasks
- Filled the volumetric flasks to the indicated line with distilled water
- The concentration of the adenosine stock solution was determined to be 3.06 mM
- The concentration of the inosine stock solution was determined to be 4.70 mM
- The amount of stock solution required was determined using an equation derived from M1V1=M2V2 formula: Amt. of Stock Sol.= [10 mL(desired dilution concentration)/(stock solution concentration)]
- A sample from the stock solution was pipetted into a clean 10 mL volumetric flask.
- Distilled water was added up to the indicated line of the volumetric flask to yield a 10 mL solution.
- This process was repeated for each dilution using a clean volumetric flask.
- An absoprtion spectrum of a cuvette filled with distilled water was collected
- 1 mL samples of a dilution were pippetted into a clean cuvette
- The cuvette was placed in the UV-Vis instrument and analyzed
- The cuvette was rinsed 3x with distilled water, and the process was repeated
- There was not enough time for all lab groups to complete their UV-Vis spectra collection, thus only data for Adenosine was collected on this day.
- The stock solutions and dilutions were stored for use on the next day.