User:Howard Boland/Notebook/Art from Synthetic Biology/2011/01/27
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I am exploring suitable media for growing nanomagnetic bacteria. I will need to make both liquid broth and agar for plates. For the broth I used ready-made Brucella broth and I also prepared Flask Standard Media. I used Kligler Iron Agar for the plates. I also tried to streak out the bacteria on standard agar plates.
28g/L, for 200ml this yields 28g/(1000ml/200ml) = 28g/5 = 5.2g
For the flask standard media, the last step requires ferric citrate. We did not have this in any of the labs so I tried to make some using ferric chloride and citric acid. A patent states that this is produced using 1:1 mole. Ferric Chloride (hexhydrate), a yellow toffee-like substance weighs 270.3g/mole whilst Citric Acid a sugary powder weighs 210.14g/mole.
To make 10ml 1M ferric citrate
Stir to mix
Flask Standard Media (FSM)
The protocol for FSM was suggested in the paper (Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor) for growing nanomagnetic bacteria. To make 1L FSM Media
Adjust PH to 7.0
I distributed the medium in 4 bottles with 200ml media each and added the remaining to small flask using 10ml media in each.
Autoclave at 121ºC for 15 minutes
Kligler Iron Agar
I am experimentally using this agar to see if it produces any growth, the bottle suggests using 500g powder per 9L. To make 200ml agar, 500g/9L = 500g/9000ml => 500g/ (9000ml/200ml) = 500g/45 = 11.1g