User:Howard Boland/Notebook/Art from Synthetic Biology/2010/09/15

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End Overnight Growth pUA66katE

All samples grew but I did a late pickup.

  1. The samples were left in 4C, for 2 hours
  2. Glycerol stock samples were taken
  3. The remaining cells in each of four bottles (10ml) centrifuged for 10 minutes.
  4. The supernatant was removed
  5. The four bottles stored at -20C.

Start Overnight Growth pUA66kat

Two samples were grown based on the new plates transformed with confirmed pUA66katE plasmids.

  1. Two colonies where picked from the plates
  2. Inoculated each colony in 10ml LB-broth containing (10ul 50mg/ul Kanamycin)
  3. The samples where placed in a shaker for overnight growth.