User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/12

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Objectives

  1. Run fluorescence data for lysozyme at pH=8
  2. Create stock solutions for use on 10.18.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 10/18/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data

101216 Lys Emis v time pH 8.png

Figure 1 Maximum emission data in a solution of pH 8.

101216 Lys Int v time pH 8.png

Figure 2 Integrated fluorescence data in a solution of pH 8.

101216 Lys Fluor Summary pH 8.png

Figure 3 Full emission spectra for important time points: t=0, 3, 21, 39, 159, 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=3 min, this maximum has shifted up and to the right.
  3. Until t=39 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=39 min, the maximum begins increasing again.
  5. At t=159 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 10.18.2016
101816 UVvis Conc Fruc .125mM.png
Table 1 UV-vis solutions made for [Fructose]=.125mM with silver

101816 UVvis Conc Fruc .25mM.png
Table 2 UV-vis solutions made for [Fructose]=.25mM with silver