User:Gabriel Berriz/Notebook/ICBP45/Overview/Anomalous fields
Below is a summary of fields (or, more precisely, field/channel pairs) for which the mean readout is negative. The numbers q0-q4 represent the minimum, 1st quartile, median, 3rd quartile, and maximum readout values for the field/channel. The fact that q3 is negative in all cases suggests that the common cause for all the anomalies is an artefactually high background. JM confirmed this guess on a couple cases by direct visual inspection of the corresponding image files (20110421_184B5/GF2/E08/4/GF2_E08_4_w685.tif, 20100925_HCC1806/CK1/B07/1/CK1_B07_1_w685.tif). In both cases, regions of anomalously high intensity (from extraneous particles) in the 685 channel coincided with some of the pixels in the background mask (which is computed from the 460 channel, where the artefact does not show).
NB: these fields stood out for having negative intensities, but it is quite likely that the signal is artefactually low, even if still positive, due to an inappropriately high estimate of the background.
scans/linkfarm/20100925_HCC1806/CK1/A01 3/CK1_A01_3_w685.tif q0: -544 (min) q1: -437 q2: -408 (mdn) q3: -371 q4: 5208 (max) scans/linkfarm/20100925_HCC1806/CK1/B07 1/CK1_B07_1_w685.tif q0: -220 (min) q1: -151 q2: -138 (mdn) q3: -122 q4: 5717 (max) scans/linkfarm/20101202_SKBR3/GF3/B06 4/GF3_B06_4_w685.tif q0: -4103 (min) q1: -3707 q2: -3592 (mdn) q3: -3462 q4: 45867 (max) scans/linkfarm/20101221_HCC1428/GF4/E08 4/GF4_E08_4_w685.tif q0: -1021 (min) q1: -910 q2: -861 (mdn) q3: -811 q4: 29952 (max) scans/linkfarm/20101221_HCC1428/GF4/G08 1/GF4_G08_1_w685.tif q0: -1474 (min) q1: -1056 q2: -1018 (mdn) q3: -956 q4: 46825 (max) 2/GF4_G08_2_w685.tif q0: -1102 (min) q1: -727 q2: -686 (mdn) q3: -644 q4: 49676 (max) scans/linkfarm/20101221_HCC1428/GF4/H08 1/GF4_H08_1_w685.tif q0: -1083 (min) q1: -679 q2: -635 (mdn) q3: -581 q4: 56017 (max) 4/GF4_H08_4_w685.tif q0: -599 (min) q1: -527 q2: -485 (mdn) q3: -434 q4: 10030 (max) scans/linkfarm/20101221_HCC1428/CK1/B04 3/CK1_B04_3_w685.tif q0: -1258 (min) q1: -963 q2: -910 (mdn) q3: -858 q4: 46686 (max) scans/linkfarm/20101221_HCC1428/CK1/C03 4/CK1_C03_4_w685.tif q0: -1700 (min) q1: -1343 q2: -1283 (mdn) q3: -1196 q4: 43611 (max) scans/linkfarm/20101221_HCC1428/CK1/H04 3/CK1_H04_3_w685.tif q0: -2012 (min) q1: -1862 q2: -1759 (mdn) q3: -1664 q4: 57500 (max) scans/linkfarm/20101221_HCC1428/CK1/H06 4/CK1_H06_4_w685.tif q0: -1565 (min) q1: -1095 q2: -1053 (mdn) q3: -998 q4: 56494 (max) scans/linkfarm/20110317_MDAMB231/CK1/H05 3/CK1_H05_3_w685.tif q0: -944 (min) q1: -743 q2: -675 (mdn) q3: -562 q4: 16039 (max) scans/linkfarm/20110415_MCF12A/GF2/A11 4/GF2_A11_4_w685.tif q0: -3469 (min) q1: -3190 q2: -3030 (mdn) q3: -2746 q4: 21408 (max) scans/linkfarm/20110421_184B5/GF2/E08 4/GF2_E08_4_w685.tif q0: -2522 (min) q1: -2235 q2: -2096 (mdn) q3: -1896 q4: 1060 (max)