User:Gabriel Berriz/Notebook/ICBP45/Overview/Anomalous fields
Below is a summary of fields (or, more precisely, field/channel pairs) for which the mean readout is negative. The numbers q0-q4 represent the minimum, 1st quartile, median, 3rd quartile, and maximum readout values for the field/channel. The fact that q3 is negative in all cases suggests that the common cause for all the anomalies is an artefactually high background. JM confirmed this guess on a couple cases by direct visual inspection of the corresponding image files (20110421_184B5/GF2/E08/4/GF2_E08_4_w685.tif, 20100925_HCC1806/CK1/B07/1/CK1_B07_1_w685.tif). In both cases, regions of anomalously high intensity (from extraneous particles) in the 685 channel coincided with some of the pixels in the background mask (which is computed from the 460 channel, where the artefact does not show).
NB: these fields stood out for having negative intensities, but it is quite likely that the signal is artefactually low, even if still positive, due to an inappropriately high estimate of the background.
scans/linkfarm/20100925_HCC1806/CK1/A01
3/CK1_A01_3_w685.tif
q0: -544 (min)
q1: -437
q2: -408 (mdn)
q3: -371
q4: 5208 (max)
scans/linkfarm/20100925_HCC1806/CK1/B07
1/CK1_B07_1_w685.tif
q0: -220 (min)
q1: -151
q2: -138 (mdn)
q3: -122
q4: 5717 (max)
scans/linkfarm/20101202_SKBR3/GF3/B06
4/GF3_B06_4_w685.tif
q0: -4103 (min)
q1: -3707
q2: -3592 (mdn)
q3: -3462
q4: 45867 (max)
scans/linkfarm/20101221_HCC1428/GF4/E08
4/GF4_E08_4_w685.tif
q0: -1021 (min)
q1: -910
q2: -861 (mdn)
q3: -811
q4: 29952 (max)
scans/linkfarm/20101221_HCC1428/GF4/G08
1/GF4_G08_1_w685.tif
q0: -1474 (min)
q1: -1056
q2: -1018 (mdn)
q3: -956
q4: 46825 (max)
2/GF4_G08_2_w685.tif
q0: -1102 (min)
q1: -727
q2: -686 (mdn)
q3: -644
q4: 49676 (max)
scans/linkfarm/20101221_HCC1428/GF4/H08
1/GF4_H08_1_w685.tif
q0: -1083 (min)
q1: -679
q2: -635 (mdn)
q3: -581
q4: 56017 (max)
4/GF4_H08_4_w685.tif
q0: -599 (min)
q1: -527
q2: -485 (mdn)
q3: -434
q4: 10030 (max)
scans/linkfarm/20101221_HCC1428/CK1/B04
3/CK1_B04_3_w685.tif
q0: -1258 (min)
q1: -963
q2: -910 (mdn)
q3: -858
q4: 46686 (max)
scans/linkfarm/20101221_HCC1428/CK1/C03
4/CK1_C03_4_w685.tif
q0: -1700 (min)
q1: -1343
q2: -1283 (mdn)
q3: -1196
q4: 43611 (max)
scans/linkfarm/20101221_HCC1428/CK1/H04
3/CK1_H04_3_w685.tif
q0: -2012 (min)
q1: -1862
q2: -1759 (mdn)
q3: -1664
q4: 57500 (max)
scans/linkfarm/20101221_HCC1428/CK1/H06
4/CK1_H06_4_w685.tif
q0: -1565 (min)
q1: -1095
q2: -1053 (mdn)
q3: -998
q4: 56494 (max)
scans/linkfarm/20110317_MDAMB231/CK1/H05
3/CK1_H05_3_w685.tif
q0: -944 (min)
q1: -743
q2: -675 (mdn)
q3: -562
q4: 16039 (max)
scans/linkfarm/20110415_MCF12A/GF2/A11
4/GF2_A11_4_w685.tif
q0: -3469 (min)
q1: -3190
q2: -3030 (mdn)
q3: -2746
q4: 21408 (max)
scans/linkfarm/20110421_184B5/GF2/E08
4/GF2_E08_4_w685.tif
q0: -2522 (min)
q1: -2235
q2: -2096 (mdn)
q3: -1896
q4: 1060 (max)