User:Eric M. Walters/Notebook/Spring 2012/2012/05/29

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Colony PCR of transformants

  • Note: this was all lost due to OWW losing it when I saved, here's from memory:
  • Had 18/9 colonies on KO/wt plates, screened 14 (12/2) with the outermost flanking primers (v1 upF/dnR)
  • Ran the reaction overnight, to run gel tomorrow