User:Eric M. Walters/Notebook/Spring 2012/2012/05/30

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Colony PCR from flanking primers

30 colony PCR 1.jpg

This is a gel of the PCR run yesterday. This could be false negatives, since if there were only one copy of the barcode sequence inserted, it would likely not show up. So today I'm repeating it with the BC-F primer at 52°C.

10 uL GoTaq reactions

  • 5uL GoTaq mastermix
  • .5 uL BC-F
  • .5 uL DnRv1
  • 4 uL water

Colony PCR

Pflegerlab 2012-05-30 17hr 10min internal colony PCR.jpg