User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/14

Absorbance vs Tris Concentration

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Objective To continue the experiment performed on 02/01/12 to determine the effects of various concentrations of tris buffer on AuNP absorbance. Description Take UV-vis data for various dilutions of tris buffer (pH 10.9) 1 M 500 mM 200 mM 100 mM 75 mM Data Graph of absorbance vs wavelength for AuNP and different dilutions of tris While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values. Graph of peak absorbance vs -log(ionic strength of tris buffer solution). Graph of peak wavelength vs -log(ionic strength of tris buffer solution). Notes