User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/08

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To make a solution of AuNP.


  1. Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 20 mL with water.
  3. Place in oven at 74°C.
  4. Leave solutions in oven for 3.5 hr.
  5. Continue to take UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution over time.
  6. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution over time.


  • Graph of absorbance vs wavelength data over time for 70 Au/BSA ratio solution prepared on 1/31/12:
Absorbance vs wavelength over time, 1 wk.jpg


UV-vis data for AuNP + 50 mM Tris (pH 10.1) solution (made on 02/01/12) could not be taken, as the AuNP precipitated out of solution.

Preciptated AuNP.png