User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/07

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Objective

To determine the effect of Tris buffers of different pH on a AuNP solution.

Description

  1. Take UV-vis data of 70 Au/BSA ratio solution prepared on 01/31/12.
  2. Take UV-vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following pH values of 50 mM Tris:
    • pH 7.5
    • pH 10.1
    • pH 11.85

Data

  • Graph of absorbance vs wavelength:
Absorbance vs wavelength 2-7-12.jpg


There appears to be a small shift toward UV and slightly increased absorbance with high pH values.

Notes

The pH meter used to determine the pH values of 50 mM tris solutions was having difficulty being calibrated.