Objective
- Remake Au:BSA solutions with ratios of 60, 90, 120, 150
- Make Au:AA solutions with same ratios.
- Prepare 50mL of 250μM of three different kinds of AA
- Test pH of AA solutions
- Measure UV-Vis of AA stock solutions
Description
- BSA Solutions- Amount of BSA needed for each ratio
Calculation: 1mL HAuCl4 * 1L/1000mL*2.5mmol/1L * 1 mol/1000mmol = 2.5*10^-6 mol HAuCl4
2.5*10^-6 mol HAuCl4/Ratio*10^6μmol/ 1 mol * 1L/ 250μmol = Volume BSA
- Amino Acid Stock Preparation
Data
- UV-Vis data for AA Stock Solutions
Tryptophan has a peak at 279 with an intensity of 1.21 and a peak at 217 with an intensity of 4.038.
Excitation at 279
Peak at 375.5
Notes
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