User:Dominique Brager/Notebook/481 Notebook/2017/08/30

Biomaterials Design Lab

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Objective Remake Au:BSA solutions with ratios of 60, 90, 120, 150 Make Au:AA solutions with same ratios. Prepare 50mL of 250μM of three different kinds of AA Test pH of AA solutions Measure UV-Vis of AA stock solutions Description BSA Solutions- Amount of BSA needed for each ratio Calculation: 1mL HAuCl4 * 1L/1000mL*2.5mmol/1L * 1 mol/1000mmol = 2.5*10^-6 mol HAuCl4 2.5*10^-6 mol HAuCl4/Ratio*10^6μmol/ 1 mol * 1L/ 250μmol = Volume BSA Amino Acid Stock Preparation Data UV-Vis data for AA Stock Solutions Tryptophan has a peak at 279 with an intensity of 1.21 and a peak at 217 with an intensity of 4.038. Tryptophan Fluoresence Excitation at 279 Peak at 375.5 Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.