User:Dhea Patel/Notebook/Phosphorylation/2012/07/12

Phosphorylation Lab

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Objective Learn how to maintain an OpenWetWare Notebook. Description Solubility tests were run on RuATP. After determining the solvents that dissolved the RuATP, 6 crystal growth systems were set up, using 10mg of RuATP per system. The RuATP was placed in a clean, small vial and filled with just enough solvent to dissolve all the RuATP. The small vial, uncovered, was placed in a clean, larger vial and filled with just enough precipitant so that the small vial did not float in the larger vial. The system was placed in an area where it would not be disturbed and covered securely with the large vial's lid. Solvent Precipitant H2O Acetone H2O Chloroform Methanol Acetone Methanol Chloroform Ethanol Acetone Ethanol Chloroform Data RuATP was soluble in: 0.1M TEAB Methanol Ethanol Pyridine Water Iso-butyric Acid RuATP was partially soluble in: Triethanolamine Isopropanol N,N-Diethylformamide RuATP was not solube in: Dichloromethane Chloroform Acetone 2-Butanone Ethyl Acetate Tetrahydrofuran Acetonitrile Triethylamine Notes This area is for any observations or conclusions that you would like to note.