User:Dhea Patel/Notebook/Phosphorylation/2012/06/29

Phosphorylation Lab

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Objective Learn how to maintain an OpenWetWare Notebook. Description Running UV vis on ATP. 100mM ATP (disodium ATP) = 0.1M ATP MW: 605.24g/mol mass: 0.605g/10mL We tried to create a serial dilution: 1mL 100mM ATP:9mL H2 = ATP C1 = 0.01M ATP 0.001M ATP = ATP C2 0.0001M ATP = ATP C3 0.00001M ATP = ATP C4 0.000001M ATP = ATP C5 0.0000001M ATP = ATP C6 0.00000001M ATP = ATP C7 0.000005M ATP = ATP 4.5 0.0000025M ATP = ATP 4.25 0.0000005M ATP = ATP 5.5 0.00000025M ATP = ATP 525A 0.00000005M ATP = ATP 6.5 0.000000025M ATP = ATP 6.25 ATP 5.5B ATP 5.25B ATP 6B ATP 6.5B ATP 6.25B 0.000000005M ATP = ATP 75B 0.0000000025M ATP = ATP 725B The graphs from this data did not provide significant information. lower limit 1μL in 100mL → 0.00001 M ATP 1 1μL in 50mL → 0.00002 M ATP 2 too high 1μL in 10mL → 0.0001 M ATP 3 too high stock → 0.1 M ATP STK 2μL in 50mL → 0.00004 M ATP 4 too high 3μL in 50mL → 0.00006 M ATP 5 2.5μL in 50mL → 0.00005 M ATP 6 1μL in 10mL → 0.0001 M ATP 3A The data from this UV vis indicated either contamination or error so another UV vis was run with new samples. 1μL in 100mL → 0.00001 M A1 1μL in 50mL → 0.00002 M A2 2μL in 50mL → 0.00004 M A3 2.5μL in 50mL → 0.00005 M A4 2.3μL in 50mL → 0.000046 M A5 2.4μL in 50mL → 0.000048 M A6 1.3μL in 50mL → 0.000026 M A7 1.5μL in 50mL → 0.000028 M A8 1.7μL in 50mL → 0.000034 M A9 3μL in 100mL → 0.00003 M A10 1μL in 10mL → 0.0001 M A11 3μL in 50mL → 0.00006 M A12 Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note.