User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/08/01
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The Daily Grind
trees were placed into 50 mL polypropylene tubes containing washing solution (0.1 M potassium phosphate buffer, pH 7.0) and sonicated for 10 min at 22.5 kHz in an ultrasonic cell disrupter (Misonix Inc., Atlantic Beach, NY, USA ) (6). The resulting bacterial suspension was centrifuged at 30,000 g for 15 min at 4oC. The pellet was resuspended in 1.5 mL of washing solution and further concentrated by centrifugation at 30,000 g for 15 min at 4oC. Bacteria were resuspended in 200 μL of washing solution and frozen at -20oC until processing for DNA extraction. Its from Lambais et al (http://www.sciencemag.org/content/312/5782/1917.full.pdf). I plan to use 30 mL of liquid per 50 mL tube, and add 5 drops of Tween 20 per litre.