User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/24

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RE digest KAH60 and KAH126

Reagent Volume Expected:
1. KAH60/pcVN = 6787
2. KAH126/MV2 = 6439
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
dH2O 9.5
  15 μL --> 37°C/ 15 min.


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