User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/11

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Insert gel check

Reagent Volume Expected:
1. Plasmid 1-10 = 1814, 5450
2. Plasmid 11 = 5191
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
Pst1 1.0
dH2O 9.5
  15 μL --> 37°C/ 15 min.