User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/11

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Insert gel check Reagent Volume Expected: 1. Plasmid 1-10 = 1814, 5450 2. Plasmid 11 = 5191 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 3.0 μL 10X buffer 1.5 Pst1 1.0 dH2O 9.5   15 μL --> 37°C/ 15 min.