User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2013/11/12

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Objective

Make Hemoglobin and Lysozyme nano particles

Description

  1. Lysozyme AuNP and Hemoglobin AuNP
    1. Determine the total amounts of materials you will need for the following samples using the same procedures as the last two times we made protein-AuNPs (final Au concentration of 0.25mM and a final volume of 5mL)
      1. Lysozyme
        1. 5
        2. 10
        3. 15
        4. 20
        5. 25
        6. 30
        7. 35
        8. 40
        9. 45
        10. 50
      2. Hemoglobin
        1. 30
        2. 50
        3. 70
        4. 90
        5. 110
        6. 130
        7. 150
        8. 170
        9. 190
        10. 210
    1. Make your stock solutions
    2. Make your samples in small test tubes and cap with aluminum foil
    3. Heat your samples at 80C for 4 hours

Data

The stock solutions we made with the following concentrations

  1. Lysozyme
    1. 34.6 mg in 25mL of water ---> 96.7 μM
  2. Hemoglobin
    1. 200 mg in 25mL of water ---> 117.7 μM
  3. Gold
    1. Need 50mL of 2.5mM
    2. 57.1mg of HAuCl4·3H2O in 50mL water --> 2.898 mM