User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2013/11/06

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Objective

Analysis and synthesis of more protein-AuNP materials. This week we made Hemoglobin-AuNPs and Lysozyme-AuNPs.

Description

  1. Before any measurement, take note of physical appearance of your samples (taking pics with cell phones helps here)
  2. Atomic Absorption
    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio
  3. UV Vis
    1. centrifuge 1mL of your sample (use the small centrifuge in 207) 30min at max rpm
    2. Take measurements of your samples

Results

Here is the UV-Vis spectra for Myoglobin at different ratios with Gold MyoglobinAuNP UV-Vis.png

Here is the UV-Vis spectra for Lysozyme at different ratios with Gold LysozymeAuNP UV-Vis 1.png

Here is the atomic absorption spectra for Myoglobin and Lysozyme MyoglobinAuNP AA.png

LysozymeAuNP AA 1.png