User:Cosette Brianna Taggart/Notebook/Experimental/2016/10/04

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October 4, 2016 : Fluorescence IV

Objective

  1. Collect fluorescence data for lysozyme at pH 7
  2. Make stock solutions for UV-Vis on 10.5.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 3 minutes

Stock Solutions for 10.05.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

SUMMARY DATA AND FIGURES

Summary Data at 15 minute increments
Time(minutes) Maximum Emission Integrated Intensity
0 352.5 5731.515152
15 424 3343.434928
30 425 3232.475321
45 430 3742.839387
60 424 4493.33215
75 426 5089.910289
90 431.5 5752.578004
105 433.5 6376.874369
120 427.5 6801.214915
135 423.5 7361.003999
150 431 7954.919134
165 427 7987.84818
180 426.5 8200.486107

IIvT100416.png

MEvT100416.png

100416 Full Fluor Lys pH 7.png