User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/28

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

September 28, 2016 : Ocean Optics III

Objective

  1. Run Ocean optics on sample prepped today.

Description

Ocean Optics

  1. Sample was prepped according to Lysozyme prep seen in data
  2. Sample was placed into Ocean Optics Machine
  3. Machine was run for 3 hours collecting data every 2 minutes

Data

Stock solutions

  1. Lysozyme
    1. concentration = 210 μM
  2. AuCl3
    1. concentration = 4.27 mM

Instructions for making sample for ocean optics

  1. pH 6
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 176 μL AuCl3
      2. 3 μL 1M HCl
      3. 179 μL Lysozyme
      4. 2642 μL Water


Summary Data at 30 minute increments
Time(minutes) Maximum Absorbance Maximum Wavelength(nm)
0 0.13438 449.84
30 0.14248 449.84
60 0.28637 539.32
90 0.45071 533.76
120 0.55944 533.06
150 0.63036 533.76
180 0.67341 530.62


MAvT pH6.png

MWvT pH6.png

092816 Abs Wave pH 6.png