- To find the sensitivity of the fluorometer using Ru(bpy)(phen) in acetonitrile.
First, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM:
||Amount of Ru(bpy)(phen)
||Amount of acetonitrile
||Final Concentration in Cuvette
The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed:
The curves were integrated in Excel and the following areas under the curves were found:
These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument:
The calculated integrated intensities for all data collected from the class was graphed in the same way:
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