Entry title
Objective
Biomineralization of gold nanoparticles while keeping the solution at 80°C the entire time. Repeat yesterday's procedure, but use distilled water instead of buffer.
Description
- Mix 8mL distilled water, 1mL 15.4μM BSA, and 580μL of 4.3mM Chloroauric acid.
- Take 1mL of this solution and transfer it to a quartz cuvette, and place the cuvette in the Shimadzu UV-2550 spectrophotometer at 70°C.
- Take a spectrum from 200nm-800nm of this every half hour for 3.5 hours.
- Put the rest of the solution in the oven set to 80°C for about 3.5 hours.
Data
- The Absorbance against wavelength taken of the solution at several times:
- Zoomed in on wavelength 550 nm:
- Graph of Absorbance against time at 550nm:
No cell growth was observed on the plates prepared 9/27/11.
Notes
- The final concentration of BSA is 1.61μM.
- Calculation:(1mL/9.58mL)*15.4μM
- The final concentration of Chloroauric acid is 0.26mM.
- Calculation: (.58mL/9.58mL)*4.3mM
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