User:Carly M. Montanero/Notebook/CHEM-571/2013/10/23

Biomaterials Design Lab: Fall 2013

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Objective To take the spectra of the pepsin-AuNP samples made yesterday To make new HRP and Myoglobin samples Procedure Protein-AuNP analysis Take a picture and note physical description of each ratio Place 1mL of each ratio in an 1.5mL eppendorf tube. Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.) Take a UV-Vis spectrum of each sample (Be considerate of other users here) After taking the spectrum, save the sample in a fresh eppy tube. Synthesis of HRP-AuNPs and Hemoglobin-AuNPs Into test tube that will contain a total of 5mL add Au solution such that the final concentration is 0.25mM For Myoglobin make the following solutions to reflect an Au:Protein ratios 30 50 70 90 110 130 150 170 190 210 For HRP make the following solutions to reflect an Au:Protein ratios 230 250 270 290 310 330 350 370 390 410 Water up to 5mL Figures How solutions were made: HRP UV-Vis Myoglobin UV-Vis Notes Stock Solutions Gold 49.3 mg HAuCl4·3H2O in 50mL water ---> 2.50mM HRP 6.6 mg HRP in 100 mL water ---> 1.5μM Myoglobin 28.7mg myoglobin in 100mL water ---> 16.9μM