User:Carly M. Montanero/Notebook/CHEM-571/2013/10/22

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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Procedure

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Source: Dr. Hartings

Data

  • Solutions Made:

Solutions 10.22.2013.png


  • UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant

CMJ UV-VIS solutions 10222013.png


  • Pepsin UV-Vis

11.1.2013 cmj UVVis Pepsin.png


  • HRP UV-Vis

CHEM571 10.23.13 HRPUvVIS.png