User:Brian P. Josey/Notebook/2009/11/13
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PCR and Some Ideas
I managed to read some more from Molecular Cloning last night about the PCR. Despite being such a large book that is dense with material, it is surprisingly easy to read and interesting. I will update some notes a little later on today or over the weekend. I also want to gather my ideas together onto a couple of pages in the notebook.
10X PCR Buffer:
Just for the sake of practice, I made a generic PCR spreadsheet. I designed it to include concentrations of Mg+2 to go from 1.0 mM to 5.0 mM.
Anthony Salvagno 16:07, 13 November 2009 (EST): Looks great! Some quick notes...
With all that said I think you are off to a great start. I think you should set up a PCR plan maybe at some point this weekend and do it on Monday. Pick 2 primers (say the F834-dig and the R1985) and do different MgCl2 concentrations ranging from 1-5mM in different tubes.