User:Benjamin D. Eachus/Notebook/Phosphorylation/2012/02/08

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  • Today's work is a continuation of Jordan's work
  • The Ru(bpy)2 (phen-NH2)](PF6)2+ATP in DMF (orange) mixture was filtered using a vacuum flask. The solid was captured on the filter paper and the liquid mixture was collected in the flask. Small samples of the solid were scraped off the paper and dissolved in one of 4 solvents; Acetone, Ethanol, Methanol and H2O. Water was the effective solvent and the remaining filter paper containing the solid was immersed in H20 in a beaker and allowed to sit.
  • The liquid sample Ru(bpy)2(phen-NH2)](PF6)2 + ATP was triturated in ether where it formed a fine precipitate and cooled on ice for approx. 45 min. The triturated mixture was then filtered using a vacuum flask where the Ru complex with ATP was captured. The solid Ru complex with ATP was immersed in H20 in a beaker and allowed to sit.
  • A combined 50 mM of tetrabutylammonium chloride and tetrabutylammonium bromide were added to 1L of water and mixed until dissolved. Once dissolved, 100mL of the tetrabutylammonium solution was added to 100 mL of Ion Exchange Resin AGC-243. A column was prepared with glass wool. Once settled, the tetrabutyl-resin mixture was added to the column and allowed to settle for 10 min. A layer of sand was added over the resin to buffer and protect the resin layer. The tetrabutyl solution was run through the column until the rendered solution was neutral.
  • These solutions were tested here.