User:Beatriz Gimenez De C./Notebook/572/2015/03/17

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Tasks

  • Run Bradford analysis of the thermolysin samples run Wednesday 04
    • 200μM of a 1 in 4 Bradford dilution (1 mL Bradford in 3 mL of Tris/NaCl buffer)
    • 150μM of each thermolysin sample
    • 550μM of tris/NaCl buffer
  • Set up lysozyme controls for all five proteases.

Results

  • thermolysin Bradford Kinetics

Bradford kinetics 1uM thermolysin.png

Bradford kinetics 100nM thermolysin.png

Bradford kinetics 10nM thermolysin.png

Bradford kinetics 1nM thermolysin.png