User:Beatriz Gimenez De C./Notebook/571/2014/11/11

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Tasks

  • New lysozyme calibration in the range 20-100 μL
  • To run dialysis of 0.6 g/L lysozyme vs 5-45 mM CaCl2 using MWCO 3500
    • Creation of bradford calibration curve (Using 0.6 g/L lysozyme stock)
      • Lysozyme calibration curve at 578 nm

Lysozyme 578.png

    • Ca2+ ISE
CaCl2 concentration (mM) Potential measurement (mV)
5 (1) 59.1
15 (2) 70.5
25 (3) 72.2
35 (4) 75.9
45 (5) 79.1
Substance Potential measurement (mV)
Lysozyme 1 53.9
Lysozyme 2 65.6
Colloid 3 71.8
Colloid 4 78.6
Colloid 5 82.7