User:Beatriz Gimenez De C./Notebook/571/2014/11/05

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Task

  • Data analysis of CaCl2 vs Colloid
    • precipitation occurred around 25 mM of CaCl2, and the 35, 45 mM of CaCl2 solutions were fully precipitated.
    • UV-Vis and bradford analysis were made of 5 nM, 15 nM,and 25 nM colloid solutions
      • Bradford data:

11-5-14 brdford data.png

      • UV-Vis Data:

UV-VIS BSA 11-5-14.png

    • Fluorescence data was collected of all solutions
    • Ca2+ ISE was also measured for all solutions
      • Data tables:
concentration CaCl2 (mM) Potential data (mV)
5 (1) 56.9
15 (2) 68.8
25 (3) 73.7
35 (4) 76.0
45 (5) 77.0
Colloid Potential data (mV)
Colloid 1 59.5
Colloid 2 69.6
Colloid 3 72.5
Colloid 4 76.3
Colloid 5 76.4
      • Ca2+ ISE new calibration curve