Entry title
- Lab Presentation
- Primer Design
- (Amplify 300 bp exon following our intron in Fra a 1)
- Took first/last 20 sequences of exon
- (Amplify out intron for use (but this contains biobrick cloning sites so we probably won't do this)
- Took last 5 sequences in exon, first 15 sequences in intron and last 15 sequences in intron and first 5 of following exon)
- GFP Sequence Alignment
 too many mismatches in alignment
- PCR (Bet v1, Bet v 1.2, Ger 3, LTP 1)
- Set annealing temp for 67.5
- Used ~ 100 ng of genomic aribidopsis DNA/ 50 uL reaction
- Lanes (1:Ladder; 2:LTP Sense; 3: LTP Antisense; 4:Ladder; 5:Bev v1 Sense; 6:Bev v1 Antisense; 7:Ladder; 8:Bev v1.2 sense; 9:Bev v1.2 antisense; 10: Ladder; 11:Ger 3 Sense; 12:Ger 3 Antisense)
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Project name
Main project page
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