June 17 2010
- Cloning:
- We ran a gel on our pcr products from yesterday (Fra 1 antisense and sense extracted from fresh strawberries)
- Based on the gel, it doesn't seem that the sequences were amplified (Wanted ~ .3 kb and saw .1 kb)
- Reasons: primer dimers, cDNA extraction, allergen not expressed in particular strawberry
- Digest w/ Xbai1 and Pst1/ purify to ligate into V0120 (biobrick plasmid)
- Transform into E.Coli
- Artificial miRNAs
- It seems that we will probably create artificial miRNAs for silencing
- We are waiting on the intron from the pHannibal vector
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