User:Anthony Salvagno/Notebook/Research/2010/11/18/Making EDTA
So I realized today that when I made popping buffer a week ago I didn't make sure the pH was at 8.0 and so now I need to remake it so that my EDTA has a pH of 8. I will do a recipe of how much whatever I need to add to make sure that I can do it again without needing to use the pH meter.
So I have EDTA Disodium Salt Dihydrate so I can just put this in water to get EDTA. Then I will need to add either NaOH or HCl to get the pH to 8 depending on the pH of the EDTA sans stuff.
Math: EDTA FW: 372.24 and I want 50mL at a concentration of 100mM total so:
- That means I need 1.8612 g of EDTA powder.
- Measure 1.8612 g of EDTA powder and put it in a falcon tube
- Add 25mL of DI water.
- Measure pH
- Add NaOH until pH reads 8.0
- Fill to 50mL with DI water
For me, I needed to add ~.525ml of 10M NaOH to get the pH to be 8.0. The EDTA had a pH of ~4.2.