User:Anthony Salvagno/Notebook/Research/2010/10/25/More DOG Signal
Continuing from Friday
I made two samples today, one of 0.51um beads and the other of 1.0um beads both in 4M NaCl to promote surface adhesion. I'm doing DOG (derivative of gaussian) signals of the beads. I'm using 2.5V for the AOM power and am moving the z-piezo in increments of 20mV. I took pictures of my start and end positions.
Small beads (60x + 1.6x) starting position
Small beads final position
Big beads starting position.
Big beads final position
Just from watching the signal without analyzing it seems that the best signal is when I start and it gets progressively worse. Maybe the camera and the eyepiece aren't in the same focal plane, or maybe that is indicative of where the trap is. Perhaps moving the trap back some will improve this, but I'm not inclined to play with it right now.
Image of the focal plane of best signal (full power)
The signal at that focal plane.