User:Anthony Salvagno/Notebook/Research/2010/06/25/Unzipping Construct Ligation

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Procedure

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I am doing two reactions because I have 2 different concentrations of digested pBR322. According to the nanodrop I have:

Gel Extraction from yesterday - ~170ng/ul --> 102nM
old gel extraction - ~80ng/ul --> 50nM

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Ant Ligation

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