User:Anthony Salvagno/Notebook/Research/2010/06/25/Unzipping Construct Ligation

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Procedure

{{#widget:Google Spreadsheet |key=0Agbdciapt4QZdFAtdW9sWTRVYmlBY0ROM3dtTlZvSXc |width=500 |height=300 }} I am doing two reactions because I have 2 different concentrations of digested pBR322. According to the nanodrop I have:

  • Gel Extraction from yesterday - ~170ng/ul --> 102nM
  • old gel extraction - ~80ng/ul --> 50nM