User:Anthony Salvagno/Notebook/Research/2010/02/16/DNA Concentration Tethering Part 2

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This is started from yesterday.

Protocol

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Results


I would say these experiments are a resounding success. Also I think I will tether with pBR322 to get a feel for what it is like to tether for unzipping. Let's compare these results to last Fri's experiment:


Comparing the two sets of data I would trust Fri's less because the DNA isn't diluted with Popping buffer. It also seems I didn't do as good a job removing bead clumps from the first batch of experiments. I would say bead concentration wise, my latest results are in line with Fri's excluding the 1:10 dilution of DNA. That is to say the amount of beads per sample decreases each time except in 1:10 where it has even less beads (overall) than the 1:100 sample. Hmmm...