User:Anthony Salvagno/Notebook/Research/2010/02/05/Jasper DNA cleanup, nanodrop, and gel

From OpenWetWare
< User:Anthony Salvagno‎ | Notebook‎ | Research‎ | 2010‎ | 02‎ | 05
Jump to navigationJump to search

I will do a qiaquick cleanup first.

Nanodrop

  • Tube 3 - 5.3ng/ul
  • Tube 5 - 103.4ng/ul

Ok so the result I got for Cy5 labeled stuff makes sense, but not so much for the Cy3 labeled stuff. The nanodrop was working flukey anyways. I started with negative amounts of DNA because I typically measure my blank to determine what kind of day the nanodrop is going to give me. Today it was a questionable readings day. So I don't trust the Cy3 (Tube 3) readings. Because of this I didn't bother to measure the protein amounts.

Gel Results

For some reason, the camera won't connect to my laptop and I don't have the cable here with me so I can't show the image I took right now. I can however show you this little piece of awesomeness:


Now I don't know if you can tell, I had to greatly enhance the images, but the Cy5 band is much brighter than the Cy3 which indicates that there is a good amount of Cy5. The Cy3 band is about as bright as the 100bp ladder band but who knows how much there actually is based on Andy's little bit of research yesterday. Which I am eternally grateful for, so thanks Andy! Also, I don't know why Koch complains about the coloring of the gel. The image above I would say looks way better in color than inverted B&W. Props to me on the side-by-side again.

Steve Koch 22:53, 5 February 2010 (EST): You're right, I do like the color better. Whatever you did to fix the problem, I love it!
Steve Koch 22:54, 5 February 2010 (EST): You can look into getting a UV illuminator, but I guess not too urgent.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Anthony_Salvagno/Notebook/Research/2010/02/05/Jasper_DNA_cleanup,_nanodrop,_and_gel&oldid=397829"