User:Anthony Salvagno/Notebook/Research/2010/01/27/Gel Results of Jasper try 4.2
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Results
Well there are definitely bands, but who knows how much DNA I got. I will purify this stuff along with yesterdays product and then try the PCR again but with the program I did yesterday. Maybe there is something magical about that program and that extension time.
Gel 2
I am running the gel at 100V because Koch doesn't like my 160V escapades. The PCR products have been purified as well and condensed into 1 tube per type (so two tubes total).
This looks a little dimmer than normal. Also I don't know why the PCR band is longer than the 100bp band in the ladder. Lane designations:
- Ladder
- Cy3 stuff
- Cy5 stuff
Another PCR to come and gel analysis to come tomorrow.
SJK 20:40, 27 January 2010 (EST)