User:Anthony Salvagno/Notebook/Research/2009/11/25/Unzipping Construct for Doug Smith: pBR322

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  1. 2x Ladder
  2. 3x Ladder
  3. Digested pBR322

I don't think I got complete digestion yesterday as there is a feint band above the two known bands. I had to run the gel almost to the end to get good separation. In the future I will need to gel on the larger apparatus. I'll save the purification and the ligation for next Monday so I can show Brian how to do it.