User:Anthony Salvagno/Notebook/Research/2009/11/25/Unzipping Construct for Doug Smith: pBR322
- 2x Ladder
- 3x Ladder
- Digested pBR322
I don't think I got complete digestion yesterday as there is a feint band above the two known bands. I had to run the gel almost to the end to get good separation. In the future I will need to gel on the larger apparatus. I'll save the purification and the ligation for next Monday so I can show Brian how to do it.