User:Anthony Salvagno/Notebook/Research/2009/11/17/Annealing new oligos

Annealing Recipe

I use the thermal cycler which is set to melt at 95C and gradually decrease the temp to 4C over the span of a few hours.

Notes

I noticed there were some floaty things in one of the tubes so I threw it out and remixed it. I did this by using a previous mix and inviting lots of guest biologists to help make the remix. Get it?!

Original:

Remix:

That's not even my favorite song, but I found that "remix" and I had to put it on. Here is a good remix: