User:Anthony Salvagno/Notebook/Research/2009/11/17/Annealing Buffer

10x Annealing Buffer

1x Anneal Buffer is:

• 10mM Tris
• 1mM EDTA
• 50mM NaCl

I want to make a 10x stock for easy addition to reactions. My final solution volume will be 500ml, so here is what I need:

1. 100mM Tris
2. 10mM EDTA
3. 500mM NaCl
4. Rest with H2O

I have a 100x Concentrate of TE buffer (Tris- EDTA) and 4M NaCl. So here is my recipe:

• 50ml 100x TE
• 62.5ml 4M NaCl
• 387.5ml H2O (autoclaved and distilled)