User:Anthony Salvagno/Notebook/Research/2009/11/03/Unzipping Construct Ligation Preplanning

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Run at 16C with ___nM additions of Adapter duplex every 30' until adapter is equimolar with anchor and plasmid.

After the reaction, I will gel extract the top band if there is one. I think I will do this reaction as a small reaction first (trial run) this way I don't lose all my plasmid DNA in one shot. Then if it looks promising I will ligate again but with all of it. Comments?


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