User:Anthony Salvagno/Notebook/Research/2009/09/03/Ligation of Sap Cap and Sap pBS

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Results

Now I will have no clue if this actually worked or not, but if it did I should have equal numbers of capped pBS with a SapI overhang. I ran the DNA through a Qiagen PCR cleanup kit. I eluted in 30uL of EB. Nanodrop results:

  • 19.6ng/uL - tube 1
  • 22.4ng/uL - tube 2

I want to just combine the two tubes but will wait on that.